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The presence of lectins in Robinia pseudoacacia root-tip extracts was demonstrated by horse erythrocyte agglutination and aggregation of (neo)glycoprotein-coated beads. Two lectins were purified from a crude protein extract by ion-exchange and affinity chromatography. A mixture of lectins was obtained by cation-exchange chromatography on a CM-Trisacryl column. Robinia pseudoacacia root agglutinin...
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