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Free chitosan, 2g/100g mycelia from Gongronella butleri and 6.5g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45°C for 13h and 0.35M acetic acid at 95°C for 5h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11M NaOH at 45°C for 13h and 0.35M acetic acid at 95°C for 5h and then extracted the total chitosan,...
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