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A set of 12 compounds of different chemical nature has been established to characterise RPLC columns on the basis of a polarity retention model previously developed: logk=(logk)0+p(PmN−PsN). This model allows the calculation of the retention factor (k) of any non-ionized compound using one parameter which describes the polarity of the solute (p), another one for the polarity of the mobile phase...
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