This chapter contains sections titled:
Electrochemical and Electrochemiluminescent Arrays
DNA/Enzyme Biocolloids for LC‐MS Toxicity Screening
Alternative Arrays and Other Novel Approaches
Summary and Future Outlook
Simultaneous optical and voltammetric detection of bioactivated genotoxicity is reported for the first time employing ultrathin films of DNA, model metabolic enzymes, and electrochemiluminescence (ECL) generating metallopolymer [Ru(bpy) 2 PVP 10 ] 2+ on pyrolytic graphite (PG) electrodes. Cytochrome P450 cam and myoglobin were used as model monoxygenase enzymes to mimic...
The present study compared the relative expression pattern of 10 orthologous CYP forms from families 1–4 in cDNA panels of human and mouse fetal and adult tissues. Except for CYP1A2, all of these CYPs exhibited specific patterns of expression during mouse ontogeny, suggesting possible involvement in development. Cyp1a1 and Cyp2r1 were the only two of the orthologs to be expressed only in the E7 mouse;...
Films of human cytochrome P450 1A2 (cyt P450 1A2) and polystyrene sulfonate were constructed on carbon cloth electrodes using layer-by-layer alternate absorption and evaluated for electrochemical- and H 2 O 2 -driven enzyme-catalyzed oxidation of styrene to styrene oxide. At -0.6 V vs. saturated calomel reference electrode in an electrochemical cell, epoxidation of styrene was mediated...
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