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HPLC microchips are investigated experimentally with respect to packing density, pressure drop–flow rate relation, hydraulic permeability, and separation efficiency. The prototype microchips provide minimal dead volume, on-chip UV detection, and a 75mm long separation channel with a ca. 50μm×75μm trapezoidal cross-section. A custom-built stainless-steel holder allowed to adopt optimized packing conditions...
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