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After the great commercial success of sub-3µm superficially porous particles, vendors are now also starting to commercialize 5µm superficially porous particles, as an alternative to their fully porous counterparts which are routinely used in pharmaceutical analysis. In this study, the performance of 5µm superficially porous particles was compared to that of fully porous 5µm particles in terms of efficiency,...
In this work the isopycnic method to construct kinetic plots for SFC was used to investigate the performance limits of an SFC system when using sub-2µm fully porous particles and sub-3µm superficially porous (core–shell) particles. This isopycnic kinetic plot method for SFC was developed and tested earlier for SFC separations on native silica with pure CO 2 as mobile phase. In the current...
Accurate quantitative analysis of amino acids forms an important part of targeted metabolic profiling as deviations from normal amino acid profiles can be indicative of several inborn metabolic disorders. A variable-length method development strategy for HPLC analysis on superficially porous particle columns using UV detection is proposed for the separation of 21 primary amino acids. This strategy...
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