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Einfach und unter milden Bedingungen werden Proteine durch Thiol‐En‐Photokupplung zwischen S‐Farnesylgruppen an einer genetisch kodierbaren „CAAX‐Box“‐Tetrapeptidsequenz (A ist aliphatisch) am C‐Terminus des Proteins und Oberflächen‐Thiolen immobilisiert (siehe Schema). Auf diese Art gelingt die gerichtete kovalente Immobilisierung von Proteinen direkt aus Expressionslysaten ohne zusätzliche Reinigungs‐...
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