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The current work describes the optimisation of a screen-printed electrode (SPE) system for measurement of a variety of seafood toxins, such as okadaic acid, brevetoxin, domoic acid and tetrodotoxin. A disposable screen-printed carbon electrode coupled with amperometric detection of p-aminophenol at +300mV vs. Ag/AgCl, produced by the label, alkaline phosphatase, was used for signal measurement. ELISA...
An amperometric immunosensor for the rapid detection of human heart fatty-acid binding protein (H-FABP) in whole blood was developed. Due to its rapid kinetics in plasma, H-FABP is a useful biochemical marker for the early assessment of acute myocardial infarction (AMI). A one-step direct sandwich assay in which analyte and alkaline phosphatase (AP) labelled antibody were simultaneously added to the...
. Aptamers are artificial nucleic acid ligands that can be generated against amino acids, drugs, proteins and other molecules. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process of adsorption, recovery and reamplification. Aptamers, first reported in 1990, are attracting interest in the areas of therapeutics and diagnostics and offer themselves as ideal...
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