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Non-porous, colloidal silica particles were annealed at three different temperatures, 800, 900 and 1050°C. The adsorption of lysozyme, a probe of surface roughness, was consistent with progressively reduced surface roughness as temperature increased. The heat treated silica particles were rehydroxylated and then used to pack UHPLC columns. The cationic protein lysozyme was used to probe silanol activity,...
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