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This paper describes the preparation of a cell-sized vesicle containing small vesicles from a two-planar lipid bilayer in a triple-well device using a pulsed jet flow. First, the size distribution of the cell-sized vesicles is estimated using a double-well device wherein a separator having a hole diameter of 100, 200, or 500 μm is mounted between the wells. Moreover, the small vesicles are successfully...
This paper describes simultaneou us 16 channels ion-channel recordings through membrane proteins reconstituted in bilayer lipid membrannes (BLMs) as shown in Figure 1. Although multiple ion-channel recording is necessary for high-throughput screening (HTS) of drug [1], there are no reports of such systems as far as the authors are aware. We have developed several strategies to construct BLMs arrray...
This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers...
This paper describes a microfluidic device for a high through-put ion channel recordings, in which planar bilayer lipid membranes (BLMs) are spanning across a nanometer-sized poly(p-xylylene) (parylene) pore (Figure 1). To make the nanopore, a micrometer-sized pore of parylene was prepared by photolithography, and then parylene was additionally deposited on to the micropore with the isotropic vaporization...
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