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We prepared 0.3 or 0.15mm i.d. columns from both fused silica capillaries and planar titanium wafers with machined grooves. Both types of devices were packed with sub-two micron C18 sorbent. Chromatographic efficiency and peak capacity were tested using LC instruments with low extra column dispersion (300nL2 or 30nL2 for 0.3 or 0.15mm i.d. columns, respectively). Micro column testing in gradient mode...
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