Search results for: David C. Muddiman

Items from 1 to 20 out of 191 results

article

Development of a relative quantification method for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging of Arabidopsis seedlings

M. Caleb Bagley, Anna N. Stepanova, Måns Ekelöf, Jose M. Alonso, more

Rapid Communications in Mass Spectrometry > 34 > 6 > n/a - n/a

Rationale Mass spectrometry imaging of young seedlings is an invaluable tool in understanding how mutations affect metabolite accumulation in plant development. However, due to numerous biological considerations, established methods for the relative quantification of analytes using infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) mass spectrometry imaging are not viable...

article

Internal Energy Deposition in Infrared Matrix-Assisted Laser Desorption Electrospray Ionization With and Without the Use of Ice as a Matrix

Anqi Tu, David C. Muddiman

Journal of The American Society for Mass Spectrometry > 2019 > 30 > 11 > 2380-2391

The internal energy deposited into analytes during the ionization process largely influences the extent of fragmentation, thus the appearance of the resulting mass spectrum. The internal energy distributions of a series of para-substituted benzyl pyridinium cations in liquid and solid-state generated by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) were measured using...

article

Label‐Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Fernanda Salvato, Philip Loziuk, Eduardo Kiyota, Gabriel Silva Daneluzzi, more

PROTEOMICS > 19 > 14 > n/a - n/a

Drought is considered the major abiotic stress limiting crop productivity. This study seeks to identify proteins involved in the drought response in sugarcane stems submitted to drought stress. The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified. A total of 1204 differentially...

article

Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance

Karina Lima Reis Borges, Fernanda Salvato, Philip L. Loziuk, David C. Muddiman, more

Environmental Science and Pollution Research > 2019 > 26 > 25 > 26039- 26051

This is a report on comprehensive characterization of cadmium (Cd)-exposed root proteomes in tomato using label-free quantitative proteomic approach. Two genotypes differing in Cd tolerance, Pusa Ruby (Cd-tolerant) and Calabash Rouge (Cd-sensitive), were exposed during 4 days to assess the Cd-induced effects on root proteome. The overall changes in both genotypes in terms of differentially accumulated...

article

Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality

Anqi Tu, David C. Muddiman

Analytical and Bioanalytical Chemistry > 2019 > 411 > 22 > 5729-5743

Mass spectrometry imaging is a powerful tool widely used in biological, clinical, and forensic research, but its often poor repeatability limits its application for quantitative and large-scale analysis. A systematic evaluation of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry (IR-MALDESI-MS) repeatability in absolute ion abundances during short- and long-term...

article

CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Xiaojing Yan, Jie Liu, Hoon Kim, Baoguang Liu, more

New Phytologist > 222 > 1 > 244 - 260

Lignin is the major phenolic polymer in plant secondary cell walls and is polymerized from monomeric subunits, the monolignols. Eleven enzyme families are implicated in monolignol biosynthesis. Here, we studied the functions of members of the cinnamyl alcohol dehydrogenase (CAD) and cinnamoyl‐CoA reductase (CCR) families in wood formation in Populus trichocarpa, including the regulatory effects...

article

Discriminating normal regions within cancerous hen ovarian tissue using multivariate hyperspectral image analysis

Mahsa Akbari Lakeh, Anqi Tu, David C. Muddiman, Hamid Abdollahi

Rapid Communications in Mass Spectrometry > 33 > 4 > 381 - 391

Rationale Identification of subregions under different pathological conditions on cancerous tissue is of great significance for understanding cancer progression and metastasis. Infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry (IR‐MALDESI‐MS) can be potentially used for diagnostic purposes since it can monitor spatial distribution and abundance of metabolites and...

article

Evaluation of Digital Image Recognition Methods for Mass Spectrometry Imaging Data Analysis

Måns Ekelöf, Kenneth P. Garrard, Rika Judd, Elias P. Rosen, more

Journal of The American Society for Mass Spectrometry > 2018 > 29 > 12 > 2467-2470

Analyzing mass spectrometry imaging data can be laborious and time consuming, and as the size and complexity of datasets grow, so does the need for robust automated processing methods. We here present a method for comprehensive, semi-targeted discovery of molecular distributions of interest from mass spectrometry imaging data, using widely available image similarity scoring algorithms to rank images...

article

IR-MALDESI mass spectrometry imaging of underivatized neurotransmitters in brain tissue of rats exposed to tetrabromobisphenol A

M. Caleb Bagley, Måns Ekelöf, Kylie Rock, Heather Patisaul, more

Analytical and Bioanalytical Chemistry > 2018 > 410 > 30 > 7979-7986

There is a pressing need to develop tools for assessing possible neurotoxicity, particularly for chemicals where the mode of action is poorly understood. Tetrabromobisphenol A (TBBPA), a highly abundant brominated flame retardant, has lately been targeted for neurotoxicity analysis by concerned public health entities in the EU and USA because it is a suspected thyroid disruptor and neurotoxicant....

article

Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging

Måns Ekelöf, Jeffrey Manni, Milad Nazari, Mark Bokhart, more

Analytical and Bioanalytical Chemistry > 2018 > 410 > 9 > 2395-2402

Laser systems are widely used in mass spectrometry as sample probes and ionization sources. Mid-infrared lasers are particularly suitable for analysis of high water content samples such as animal and plant tissues, using water as a resonantly excited sacrificial matrix. Commercially available mid-IR lasers have historically been bulky and expensive due to cooling requirements. This work presents a...

article

A novel integrated strategy for the detection and quantification of the neurotoxin β-N-methylamino-l-alanine in environmental samples

Joshua Beri, Kaylie I. Kirkwood, David C. Muddiman, Michael S. Bereman

Analytical and Bioanalytical Chemistry > 2018 > 410 > 10 > 2597-2605

We describe a set of new tools for the detection and quantification of β-N-methylamino-l-alanine (BMAA) which includes a novel stable isotope-labeled BMAA standard (¹³C₃,¹⁵N₂) and a chip-based capillary electrophoresis mass spectrometry platform for separation and detection. Baseline resolution of BMAA from its potentially confounding structural isomers N-2-aminoethylglycine (AEG) and 2,4-diaminobutyric...

article

Jürgen H. Gross: Mass spectrometry: a textbook, 3rd ed.

David C. Muddiman

Analytical and Bioanalytical Chemistry > 2018 > 410 > 8 > 2051-2052

article

Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT™ reagent

Samuel R. King, Elizabeth S. Hecht, David C. Muddiman

Analytical and Bioanalytical Chemistry > 2018 > 410 > 5 > 1409-1415

The INLIGHT™ strategy for N-linked glycan derivatization has been shown to overcome many of the challenges associated with glycan analysis. The hydrazide tag reacts efficiently with the glycans, increasing their non-polar surface area, allowing for reversed-phase separations and increased ionization efficiency. We have taken the INLIGHT™ strategy and adopted it for use with O-linked glycans. A central...

article

Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae

Yeonyee Oh, Suzanne L. Robertson, Jennifer Parker, David C. Muddiman, more

Proteome Science > 2017 > 15 > 1 > 1-12

Background Fungi are constantly exposed to nitrogen limiting environments, and thus the efficient regulation of nitrogen metabolism is essential for their survival, growth, development and pathogenicity. To understand how the rice blast pathogen Magnaporthe oryzae copes with limited nitrogen availability, a global proteome analysis under nitrogen supplemented and nitrogen starved conditions was completed...

article

Perfluorinated alcohol induced coacervates as extraction media for proteomic analysis

James P. McCord, David C. Muddiman, Morteza G. Khaledi

Journal of Chromatography A > 2017 > 1523 > C > 293-299

We describe a novel method for using hexafluoroisopropanol (HFIP) induced coacervates with a variety of surfactants to extract proteins from a yeast whole cell lysate and conduct a global proteomic investigation on the extracted proteins. Yeast whole cell lysates were prepared and proteins were extracted using two workflows: 1) Proteins were extracted into the coacervate generated from the mixture...

article

Cover Image

Milad Nazari, Måns Ekelöf, Sitora Khodjaniyazova, Nathaniel L. Elsen, more

Rapid Communications in Mass Spectrometry > 31 > 22 > i - i

The cover image, by Milad Nazari et al., is based on the Research Article Direct Screening of Enzyme Activity using Infrared Matrix‐Assisted Laser Desorption Electrospray Ionization (IR‐MALDESI), DOI: 10.1002/rcm.7971.

article

Direct screening of enzyme activity using infrared matrix‐assisted laser desorption electrospray ionization

Milad Nazari, Måns Ekelöf, Sitora Khodjaniyazova, Nathaniel L. Elsen, more

Rapid Communications in Mass Spectrometry > 31 > 22 > 1868 - 1874

Rationale High‐throughput screening (HTS) is a critical step in the drug discovery process. However, most mass spectrometry (MS)‐based HTS methods require sample cleanup steps prior to analysis. In this work we present the utility of infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) for monitoring an enzymatic reaction directly from a biological buffer system with no...

article

MSiReader v1.0: Evolving Open-Source Mass Spectrometry Imaging Software for Targeted and Untargeted Analyses

Mark T. Bokhart, Milad Nazari, Kenneth P. Garrard, David C. Muddiman

Journal of The American Society for Mass Spectrometry > 2018 > 29 > 1 > 8-16

A major update to the mass spectrometry imaging (MSI) software MSiReader is presented, offering a multitude of newly added features critical to MSI analyses. MSiReader is a free, open-source, and vendor-neutral software written in the MATLAB platform and is capable of analyzing most common MSI data formats. A standalone version of the software, which does not require a MATLAB license, is also distributed...

article

IR-MALDESI method optimization based on time-resolved measurement of ion yields

Måns Ekelöf, David C. Muddiman

Analytical and Bioanalytical Chemistry > 2018 > 410 > 3 > 963-970

In the field of mass spectrometry imaging, typical experiments involve ionization directly from complex samples with no pre-ionization separation, relying on high resolving power mass analyzers to separate ions of interest. When an ion trapping step is involved in the analysis, the dynamic range of the analysis may be limited by the capacity of the ion trap, which is easily exceeded. To minimize collection...

article

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

Milad Nazari, Alexandra A. Malico, Måns Ekelöf, Sean Lund, more

Analytical and Bioanalytical Chemistry > 2018 > 410 > 3 > 953-962

Terpenes are the largest class of natural products with a wide range of applications including use as pharmaceuticals, fragrances, flavorings, and agricultural products. Terpenes are biosynthesized by the condensation of a variable number of isoprene units resulting in linear polyisoprene diphosphate units, which can then be cyclized by terpene synthases into a range of complex structures. While these...

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Search results for: David C. Muddiman

Development of a relative quantification method for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging of Arabidopsis seedlings

Internal Energy Deposition in Infrared Matrix-Assisted Laser Desorption Electrospray Ionization With and Without the Use of Ice as a Matrix

Label‐Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance

Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality

CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Discriminating normal regions within cancerous hen ovarian tissue using multivariate hyperspectral image analysis

Evaluation of Digital Image Recognition Methods for Mass Spectrometry Imaging Data Analysis

IR-MALDESI mass spectrometry imaging of underivatized neurotransmitters in brain tissue of rats exposed to tetrabromobisphenol A

Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging

A novel integrated strategy for the detection and quantification of the neurotoxin β-N-methylamino-l-alanine in environmental samples

Jürgen H. Gross: Mass spectrometry: a textbook, 3rd ed.

Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT™ reagent

Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae

Perfluorinated alcohol induced coacervates as extraction media for proteomic analysis

Cover Image

Direct screening of enzyme activity using infrared matrix‐assisted laser desorption electrospray ionization

MSiReader v1.0: Evolving Open-Source Mass Spectrometry Imaging Software for Targeted and Untargeted Analyses

IR-MALDESI method optimization based on time-resolved measurement of ion yields

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

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