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Quantitative analysis of metabolic mixtures by 1 H 1D NMR offers a limited potential for precise quantification of biomarkers, due to strong overlap between the peaks. Two-dimensional spectroscopy is a powerful tool to unambiguously and simultaneously measure a larger number of metabolite contributions. However, it is still rarely used for quantification, first because quantitative analysis...
In order to detect small variations in 13 C isotopomers concentrations, high sensitivity, accuracy and precision have to be achieved. To assess such criteria, when using 13 C NMR, 13 C bi-labelled ethanol has been proposed as a molecular probe. Advantage has been taken of the pre-established structural relationship between the peak areas of the 13 C NMR spectrum of...
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