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We investigated the impact of dietary restriction on the basal rate of somatotrope renewal in the pituitary gland. Bromodeoxyuridine (BrdU), a thymidine analog, was administered continuously for 1 week in male F344 rats at 3, 8 and 20 months of age (mo), fed ad libitum (AL) or diet restricted from 1.5 mo (DR). Combined immunostainings for BrdU and GH visualized newly formed somatotropes as well as...
We evaluated the effect of a single dose of 1.0 mg/100 g body weight of cycloheximide (CHX) on the susceptibility of hepatocytes to cell death in young (6 months old) and old (24 months old) F344 rats fed ad libitum (AL) or on dietary restriction (DR), using terminal dUTP nick end labeling (TUNEL). The proportion of TUNEL-positive hepatocytes (TPH) at baseline (without administration of CHX) was...
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