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We developed a tunnel-current based identification method of DNA / RNA sequence. This methodology is based on tunneling-current time-traces during its translocation of molecules through a nano-gap-electrode. We performed electrical measurements of DNA / RNA nucleotide translocating between gold nanogap-electrode separated by a sub-nanometer gap. Based on this determined electrical conductivity for...
Integration of waveguides and microfluidic channels enables construction of weak fluorescence detection system at a chip size. Crossed structure of a waveguide and a microfluidic channel can achieve small detection volume, which contributes to reduction of sample consumption and separation of each molecule. The decrease of defects caused by fabrication processes is indispensable because of efficient...
We developed a nano-scale electron-beam (EB) lithography procedure using a high-resistance electron-beam resist for fabrication of nano-biodevices. After a conventional EB image-development procedure, we newly added a resist-baking procedure using an EB exposure with a density of over 50 mC/cm2, and then performed a reactive-ion dry-etching. We found that the EB-baked resists were highly resistant...
We have been proposed a tunneling-current identification methodology of a DNA/RNA sequence, which is based on sequentially reading the tunneling-current across individual single-nucleotides in the sequence In this report, we determined the single-molecule electrical conductance for all kinds of deoxyribonucleotide, and ribonucleotides at the single-nucleotide level, by using gap-tunable gold nano-electrode...
We developed a simple fabrication method for a gating nanopore nanostructure, which is composed of a solid-state nanopore and embodied nanogap-electrode inside the nanopore. The method by using deep reactive-ion etching technique achieved a high-yield rate and easily controlled the pore-length for the gating nanopore device. In addition, by using the gating nanopore, we investigated single-particles...
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