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Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing machinery and the subsequent manipulation of cells. However, these workflows often involve processes such as bulk electroporation for intracellular delivery...
Genetic Manipulation
In article number 2107795, Horacio D. Espinosa and co‐workers present an automated nanopipette‐based single cell electroporation system using arrays of microwells, a deep convolutional neural network for cell nuclei localization, and hardware and software for cell–nanopipette contact detection. The system enables CRISPR/Cas9 gene editing and cell perturbation studies with unprecedented...
Introducing exogenous molecules into cells with high efficiency and dosage control is a crucial step in basic research as well as clinical applications. Here, the capability of the nanofountain probe electroporation (NFP‐E) system to deliver proteins and plasmids in a variety of continuous and primary cell types with appropriate dosage control is reported. It is shown that the NFP‐E can achieve fine...
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