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Tolaasin, a pore‐forming peptide toxin produced by Pseudomonas tolaasii, causes brown blotch disease on cultivated mushrooms. Hemolysis using red blood cells was measured to evaluate the cytotoxicity of tolaasin. To investigate the mechanism of tolaasin‐induced cell disruption, we studied the effect of temperature on the hemolytic process. At 4 °C, poor binding of the tolaasin molecules to the erythrocyte...
Enterobacter amnigenus GG0461, isolated and identified from greenhouse soils, has a high activity of nitrate uptake in the Pseudomonas agar factor medium. Although nitrate is not essential for the growth of this microorganism, it increases the growth rate. To characterize the microbial nitrate uptake, we measured the effect of chlorate on the uptake, since chlorate is an inhibitor of plant nitrate...
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