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Validated protocols for DNA purification and PCR amplification are reported for detection of Phytophthora cactorum in diseased strawberry plants. To remove PCR inhibitors, necrotic strawberry tissues were soaked in 5% alconox solution for >12 h before DNA extraction, and the extracted genomic DNA was embedded in an agarose gel chamber and subjected to electrophoresis. The purified DNA was amplified...
Root rots of grapevine in California are caused by several soilborne pathogens. While root rot in vineyards in general has been considered to be a relative minor problem, nearly all root diseases have increased in incidence and severity since the introduction of rootstocks resistant to Phylloxera. Pathogens such as Phytophthora spp. and Armillaria mellea have been known to occur for many years. However,...
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