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Identification of members of the Lactobacillus sakei group (LSG) by common phenotypic and genotypic methods is generally inadequate and time‐consuming. The objective of this study was to develop novel species‐specific primers based on sequence‐characterized amplified region (SCAR) markers using random amplified polymorphic DNA polymerase chain reaction (RAPD‐PCR) analysis. Three species‐specific...