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Objectives: To explore the role of Oct3/4, Nanog and Sox2 in regeneration of rat tracheal epithelium.
Materials and methods: An ex vivo model of rat tracheal epithelial regeneration using 5‐fluorouracil (5‐FU) was developed, to induce injury. Expression levels of Oct3/4, Nanog and Sox2 were examined using Western blot analysis, RT‐PCR or microscopically observed immunofluorescence, and cell morphological...