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To biosynthesize fluorescent Spirulina platensis (Sp) β-phycocyanin (PC) in Escherichia coli, a BLASTP search for homologs of the cpeS gene, a chromophore lyase, was performed against the Synechocystis sp. PCC 6803 (S6) proteome. A highly homologous gene, slr2049, was obtained from the S6 genome. Sites 82 and 153 in β-phycocyanin of Sp were modified by site-directed mutagenesis. Two recombinant expression...
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