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AbstractHuman collagen type III was immobilized covalently via activated carbohydrate moieties onto hydrazine-treated microtiter plates which could be used to measure von Willebrand factor (vWF) collagen binding activity (vWF:CBA) in an ELISA. Such plates were simple to prepare and remained stable at 4C and 20C for at least 2months. Samples analyzed by this system included (a) normal human vWF fractionated...