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Abstract. The pore-forming activity of tetanus toxin, its chains and fragments was studied on membrane patches from spinal cord neurons of fetal mice using the outside-out patch-clamp configuration. 1. The dichain tetanus toxin forms pores at pH5, but not at pH7.4. The elementary pore conductance is 38.41.1pS and nonselective for small cations. The open probability of the pores is voltage-dependent...