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Clostridium butyricum, a well known H2 producing bacterium, produces lactate, butyrate, acetate, ethanol, and CO2 as its main by‐products from glucose. The conversion of pyruvate to lactate, butyrate and ethanol involves oxidation of NADH. It was hypothesized that the NADH could be increased if the formation of these by‐products could be eliminated, resulting in enhancing H2 yield. Herein, this study...
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