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An efficient one-step affinity purification of bovine brain G protein βγ subunits (βγ's) is described. The βγ's, in a detergent extract of brain membranes, are first dissociated from the α subunits (α's), reassociated with decahistidine-tagged αilproduced in bacteria, and then adsorbed onto Ni2+–nitrilotriacetic acid–agarose via the histidine tag. This mild adsorption retained the high activity of...
A review is presented of selected recent topics in electrolytic and electroless gold plating for electronics applications. The topics covered include developments of non-cyanide electroplating baths for plating soft gold suitable for fabricating microbumps on silicon wafers, electroplating of hard gold and alternative materials with thermally stable electrical contact resistance and wear resistance...
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