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Fluorescent markers of cell death offer superior selectivity and sensitivity, although their applicability in detecting tissue injury under in vivo conditions remains uncertain. Here we examined whether ethidium bromide and Hoechst 33342, two widely used markers for cell necrosis and apoptosis, can be used in vivo to detect different types of cell death induced by Na,K-ATPase inhibition. Microinfusion...
Transient brain ischemia induces significant alterations in lipid structures of neuronal membranes, which are believed to result from lipid peroxidation and free radical attack. Such a membrane structural change may serve as an important histological marker of cell injury. In the present study, we examined how the dynamics of DiI/membrane incorporation may reflect early membrane metabolism and dynamic...
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