The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This paper describes a simple reusable device that hydrodynamically traps a large number of beads in an array. Guiding pillars allow us to release the trapped beads by simply reversing the flow direction. The trap and reset operations are extremely simple, robust and highly efficient. We analyzed the path of the beads in a microchannel with pillars to optimize the design of the device. We succeeded...
This paper describes a three-dimensional microfluidic axisymmetric flow-focusing device (AFFD) fabricated using stereolithography. Using this method, we can fabricate AFFDs rapidly and automatically without cumbersome alignment needed in conventional methods. The AFFDs are able to be fabricated reproducibly with a micro-sized orifice of diameter around 250 μm. Using this device, we are able to produce...
We present a highly parallel and reproducible method for reconstituting an array of lipid bilayers to analyze membrane transport. We infuse buffer/lipid/buffer solutions sequentially into a microchannel with numerous microchambers in its walls and seal each chamber by a lipid bilayer containing membrane proteins. Due to the small volume of the chamber (2 pL), membrane transport of confined fluorescent...
This paper describes a simple reusable device that hydrodynamically traps a large number of beads in an array. The device allows us to release the trapped beads by simply reversing a flow direction. The trap and reset operations are extremely simple, robust and highly efficient. With the device, we succeeded in arraying 1000 microbeads, and subsequently releasing them in a few minutes. As the demonstration...
We present a method to form monodisperse microcages encapsulating cells with poly-L-lysine (PLL) membrane. These microcages were prepared with a monolithic three-dimensional microfluidic axisymmetric flow-focusing device (3D AFFD) using internal gelation method. The production process of microcage is mild enough for cells. The microcages were sufficiently monodisperse and robust to be trapped in a...
This paper proposes a design concept and fabrication method of a planar three-dimensional (3D) microfluidic flow-focusing device (MFFD) that can produce monodisperse copolymer (EGDMA/AA) microspheres carrying surface carboxyl in a closed/open microfluidic system. The devices were made up by PDMS or SU-8 resist structures to form coaxial embedded orifices for a closed/open microfluidic system, respectively...
This work is motivated by the need for accurate positioning of both adherent and non-adherent cells into arrays that can be integrated with microfluidic channels in high-throughput screening (HTS) for drug discovery. We describe a microfluidic device that allows rapid production of uniform micro hydrogel capsules that can contain cells. Up to 1times104 hydrogel capsules can be simply formed and arrayed...
We present two achievements in this paper: (i) the preparation of monodisperse Micro-Alginate Beads (MABs) with coefficient of variation (C.V.) less than 3% by combining T-junction droplet formation with internal gelation method, and (ii) the manipulation of flow resistances in passive μ-Fluidic traps to achieve “ one bead-to-one trap” immobilization for cell array. In addition, we have succeeded...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.