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Utilization of a class of fluorescent cyanine dyes (Cy3 TM ) for the assay of gap junctional communication by the dye transfer method was examined. When compared with Lucifer Yellow (LY), a commonly used tracer, microinjected Cy3 dye was found to yield similar degrees of cell coupling. Blockade of the transfer of both tracers by 12-O-tetradecanoylphorbol-13 acetate (TPA), which is known...
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