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In this paper, we compare the cell growth results of NIH-3T3 and Neuro-2A cells over 72h on flat and honeycomb structured PLA films without and with a two-step atmospheric-pressure nitrogen-based plasma jet treatment. We developed a fabrication system used for forming of a uniform honeycomb structure on PLA surface, which can produce two different pore sizes, 3–4μm and 7–8μm, of honeycomb pattern...