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Two acidic chitinases (E.C. 3.2.1.14) were purified from embryogenic Citrus sinensis L. Osbeck cv. <Hamlin> callus tissue culture medium. The two proteins showed chitinase and chitosanase activity but no lysozyme activity. The enzyme activities decreased with decreasing acetylation of the chitin substrate. Both hydrolases were endochitinases and showed distinct differences in their digestion...