Search results for: Bartosz Kempisty

Items from 1 to 12 out of 12 results

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Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

Wiesława Kranc, Maciej Brązert, Joanna Budna, Piotr Celichowski, more

Histochemistry and Cell Biology > 2019 > 151 > 2 > 125-143

The human ovarian granulosa cells (GCs) surround the oocyte and form the proper architecture of the ovarian follicle. The ability of GCs to proliferate and differentiate in the conditions of in vitro culture has been proven. However, there is still a large field for extensive investigation of molecular basics, as well as marker genes, responsible for these processes. This study aimed to find the new...

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Supplementation of l-carnitine during in vitro maturation improves embryo development from less competent bovine oocytes

Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, more

Theriogenology > 2017 > 102 > C > 16-22

The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine,...

article

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Joanna Budna, Marta Rybska, Sylwia Ciesiółka, Artur Bryja, more

Reproductive Biology and Endocrinology > 2017 > 15 > 1 > 1-9

Background The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor...

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Polymorphic variants of MIF gene and prognosis in steroid therapy in children with idiopathic nephrotic syndrome

Monika Świerczewska, Danuta Ostalska-Nowicka, Bartosz Kempisty, Aleksandra Szczepankiewicz, more

Acta Biochimica Polonica > 2014 > 61 > 1 > 67-75

Nephrotic syndrome (NS) is the most common reason of proteinuria in children and can be caused by the pathology of renal glomeruli. Steroid therapy is typically used in this disorder. It has been shown that MIF is a cytokine which counteracts the immunosuppressive properties of glucocorticoids. The aim of this study was looking for a correlation between MIF polymorphisms and genetic susceptibility...

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Real-time proliferation of porcine cumulus cells is related to the protein levels and cellular distribution of Cdk4 and Cx43

Bartosz Kempisty, Agnieszka Ziółkowska, Hanna Piotrowska, Piotr Zawierucha, more

Theriogenology > 2013 > 80 > 4 > 411-420

The proper maturation of cumulus somatic cells depends on bidirectional communication between the oocyte and the surrounding cumulus cells (CCs). The aim of this study was (i) to investigate maturation markers, such as Cx43 and Cdk4 protein levels, and (ii) to analyze the distribution of these two proteins in CCs cultured for 44, 88, 132, and 164 hours in both separated and cumulus-enclosed oocyte...

article

Molecular basis of mechanisms of steroid resistance in children with nephrotic syndrome

Monika Świerczewska, Danuta Ostalska-Nowicka, Bartosz Kempisty, Michał Nowicki, more

Acta Biochimica Polonica > 2013 > 60 > 3 > 339-344

Steroid therapy, due to a wide range of anti-inflammatory properties of steroids, is a basic field of treatment in many human diseases including the nephrotic syndrome in children. However, not all patients respond positively to therapy which divides them into steroid sensitive (SS) and steroid resistance (SR) individuals. Many potential factors associated with steroid resistance have been identified...

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Lab-on-a-chip spectrophotometric characterization of porcine oocytes

Rafał Walczak, Patrycja Śniadek, Jan A. Dziuban, Bartosz Kempisty, more

Sensors & Actuators: B. Chemical > 2012 > 165 > 1 > 38-43

The method of quality assessment of the oocytes of breeding animals based on morphological criteria very often leads to unsuccessful in vitro fertilization and pure breeding result. In this paper we present a spectrophotometric methodology utilizing lab-on-a-chip techniques for optical and subjective characterization of porcine oocytes. A set of parameters describing the features of oocytes deduced...

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Impact of protamine transcripts and their proteins on the quality and fertilization ability of sperm and the development of preimplantation embryos

Magdalena Depa-Martynow, Bartosz Kempisty, Paweł Piotr Jagodziński, Leszek Pawelczyk, more

Reproductive Biology > 2012 > 12 > 1 > 57-72

Protamine 1 (PRM1) and 2 (PRM2) are major nuclear proteins in spermatozoa known to bind to chromatin during early spermatogenesis. The aim of this study was to evaluate the impact of protamine transcripts and proteins on human spermatozoa and their fertilization ability as well as the development of preimplantation embryos. Oocytes selected from 92 patients were fertilized in vitro (IVF) using their...

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Assessment of zona pellucida glycoprotein and integrin transcript contents in porcine oocytes

Bartosz Kempisty, Paweł Antosik, Dorota Bukowska, Marta Jackowska, more

Reproductive Biology > 2009 > 9 > 1 > 71-78

Using reverse transcription and real-time quantitative PCR analysis we evaluated the transcript levels of integrins (αL, αM, β1, and β6), CD9 and CD18 antigens as well as zona pellucida glycoproteins (pZP1, pZP2, pZP3 and pZP3α) in oocytes isolated from puberal gilts (n=20) and multiparous sows (n=20). We found significantly (p< 0.05) higher transcript contents of αL, αM, β1, and β6 integrins,...

article

The morphology of porcine oocytes is associated with zona pellucida glycoprotein transcript contents

Marta Jackowska, Bartosz Kempisty, Paweł Antosik, Dorota Bukowska, more

Reproductive Biology > 2009 > 9 > 1 > 79-85

We hypothesized that oocyte morphology may be associated with the accumulation of specific mRNAs encoding proteins responsible for the gamete fertilization ability. Therefore, the aim of the study was to evaluate the transcript levels of porcine zona pellucida (pZP1, pZP2, pZP3 and pZP4) glycoproteins in oocytes classified by a four-grade morphological scale (I-IV) accounting for either a homogeneous...

article

Distribution of 1298A>C polymorphism of methylenetetrahydrofolate reductase gene in patients with bipolar disorder and schizophrenia

Bartosz Kempisty, Anna Bober, Marta Łuczak, Piotr Czerski, more

European Psychiatry > 2007 > 22 > 1 > 39-43

We investigated the genotype frequency of methylenetetrahydrofolate reductase (MTHFR) 1298A>C polymorphism in the group of patients with bipolar disorder type I (BDI) (n=200) and schizophrenia (n=200) and in the control group (n=300). Odds ratio (OR) for patients with BD and schizophrenia in 1298CC homozygous state was 3.768 (95% CI=1.752–8.104); P=0.0003; (P=0.0006 after Bonferroni correction)...

article

Association of 677C>T polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene with bipolar disorder and schizophrenia

Bartosz Kempisty, Adrianna Mostowska, Ilona Górska, Marta Łuczak, more

Neuroscience Letters > 2006 > 400 > 3 > 267-271

Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism 677C>T has been shown to be a risk factor for psychiatric disorders. We investigated the genotype and allelic frequencies of MTHFR 677C>T polymorphism in the group of patients with bipolar disorder type I (BDI) (n=200) and schizophrenia (n=200), and in the control group (n=300). Odds ratio (OR) for patients with BD and schizophrenia...

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