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In this paper, mesoporous graphite-like carbon nitride (mpg-C3N4) combined with gold nanoparticles (Au NPs) was conducted to construct a luminol-hydrogen peroxide (H2O2) based electrochemiluminescent (ECL) immunosensor for prostate specific antigen (PSA) detection. The mpg-C3N4 exhibited large specific surface area and high porosity was synthesized from a simple precursor, which possessed abundant...
A facile and cost-effective fluorescence biosensor is constructed for the accurate and sensitive determination of DNA. The G-quadruplex-forming sequence in hairpin DNA sequence (H1) is originally locked. When target DNA is present, the hairpin structure of H1 can be unfolded and part of H1 hybridized with the target DNA to form a double-stranded DNA and a G-quadruplex. The hairpin DNA sequence (H2)...
In this study, a new and sensitive signal-off electrogenerated chemiluminescence (ECL) biosensing method for the quantification of 5-hydroxymethylcytosine in DNA (5-hmC-DNA) was developed. The method achieved simple and sensitive detection of 5-hmC-DNA based on the glycosylation of 5-hmC, combining both the amplification function of gold nanoparticles (AuNPs) and the high quenching efficiency of the...
Novel magnetic mesoporous molecularly imprinted polymers (MMIPs) with core-shell structure were prepared by simple surface molecular imprinting polymerization using tetrabromobisphenol-S (TBBPS) as the template. The MMIPs-TBBPS were characterized by fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), N2 adsorption-desorption transmission,...
A novel and simple fluorescent biosensor has been realized through the fluorescence quenching of graphite carbon nitride (g-C3N4) for mercuric ion (Hg2+) detection. In this assay, the g-C3N4 sheets which were functionalized with single-stranded DNA (ssDNA) aptamer showed strong fluorescence emission at 440nm under the excitation of 380nm in the absence of Hg2+. When added to the assay solution, Hg...
Major stumbling blocks in the recognition and detection of virus are the unstable biological recognition element or the complex detection means. Here a fluorescent sensor based on virus-molecular imprinted polymers (virus-MIPs) was designed for specific recognition and highly sensitive detection of Japanese encephalitis virus (JEV). The virus-MIPs were anchored on the surface of silica microspheres...
The reliable and accurate detection of explosives such as 2,4,6-trinitrophenol (TNP) and 2,4,6-trinitrotoluene (TNT) is in high demand for homeland security and public safety. Although extremely high sensitivity towards TNT has been demonstrated, detection of TNP remains a challenge. In this work, a fluorescent nanoscale complex composed of bis(8-hydroxyquinoline) and Al3+ ions has been prepared,...
In this paper, 3-aminobenzeneboronic acid functionalized Mn2+-doped ZnTe/ZnSe quantum dots (APBA-dQDs) were prepared. The APBA functional groups had strong binding ability with F–, resulting in the quenchment of dQDs photoluminescence (PL). Under the optimal condition, the fluorescence intensity of APBA-dQDs was related linearly to the concentration of F– in the range of 0.25–1.5µmol/L with a detection...
A disposable electrochemical immunosensor was developed for detection of aflatoxin B1 (AFB1) based on stripping voltammetric detection of copper ions released from Cu-apatite. AFB1 antibody (Ab) was firstly fixed on the gold nanoparticle (Au NPs) modified screen-printed carbon electrode (SPCE). AFB1-bovine serum albumin (AFB1-BSA) conjugate was labeled with Cu-apatite, and then competed with AFB1...
A novel sensitive electrochemical immunosensor was proposed for detection of CA125 based on ferrocenecarboxylic acid (FA), HCl-doped polyaniline (H-PANI) and chitosan hydrochloride (CS-HCl), which were used as substrate materials to generate signal and to enlarge specific surface area. The composite of these three materials (acid center compound, FA@H-PANI@CS-HCl) can be connected by hydrogen (H)...
Gold nanoparticles (AuNPs) and reduced graphene oxide (graphene) composites were synthesized via a simple one-pot approach in the presence of polyvinylpyrrolidone (PVP). Further, the Au–PVP–graphene nanocomposite was used as a new sensing material for the electrochemical detection of butylated hydroxyanisole (BHA). Because of the greatly enlarged surface area, the enhanced electron transfer rate,...
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