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Pyruvate dehydrogenase (E1), an α 2 β 2 tetramer, is the first component of the pyruvate dehydrogenase complex which catalyzes a two-step oxidative decarboxylation of pyruvic acid. To overexpress human E1 and its subunits individually, cDNAs for the mature forms of human E1α and E1β were subcloned either individually or together into a plasmid pQE-9 and expressed in Escherichia coli...