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High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography–electrospray ionization-time-of-flight mass spectrometry (LC–TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC–TOF-MS analysis; one cyclic...
Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.) Pax for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system consisting of n-butanol–ethyl acetate–water (0.6:4.4:5, v/v). The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure...
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