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Esterified acid soluble pectins from flax (Linun usitatissimum L.) were degraded either with HCl or pectin lyase. Centrifugation and 2-propanol precipitation led to the isolation of two low molecular weight polygalacturonates after acid hydrolysis of pectins. However, after pectin lyase digestion and purification by size-exclusion HPLC, 1H NMR analyses indicated that acetylated hairy regions, large...
Denatured Clostridium tyrobutyricum flagellin is rapidly degraded during incubation with commercial preparations of α-amylase, dextranase, endo-1,3,(4)-β-glucanase, chitinase and hyaluronidase indicating the presence of contaminating proteinases. Electrophoretic and solid-phase assay, with bovine serum albumin as substrate, revealed proteolytic activities in these preparations. In comparison to bovine...
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