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An LC–MS/MS/MS GHB quantification method in human serum supported by the adduct formation process with the components of the mobile phase was developed and validated. The continuous infusion of GHB made the identification of a GHB MS3 mass transition possible (185/103/85). A Luna 5μm C18 (2) 100Å, 150mm×2mm analytical column and the elution with a programmed flow of the mobile phase consisting of...
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