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We previously reported the vanadyl hyperfine couplings of VO2+–ATP and VO2+–ADP complexes in the presence of the nitrogenase Fe protein from Klebsiella pneumoniae (Petersen et al. in Biochemistry 41:13253–13263, 2002). It was demonstrated that different VO2+–nucleotide coordination environments coexist and are distinguishable by electron paramagnetic resonance (EPR) spectroscopy. Here orientation-selective...
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