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Using reverse transcription and real-time quantitative PCR analysis we evaluated the transcript levels of integrins (αL, αM, β1, and β6), CD9 and CD18 antigens as well as zona pellucida glycoproteins (pZP1, pZP2, pZP3 and pZP3α) in oocytes isolated from puberal gilts (n=20) and multiparous sows (n=20). We found significantly (p< 0.05) higher transcript contents of αL, αM, β1, and β6 integrins,...
We hypothesized that oocyte morphology may be associated with the accumulation of specific mRNAs encoding proteins responsible for the gamete fertilization ability. Therefore, the aim of the study was to evaluate the transcript levels of porcine zona pellucida (pZP1, pZP2, pZP3 and pZP4) glycoproteins in oocytes classified by a four-grade morphological scale (I-IV) accounting for either a homogeneous...
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