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Active segregation of essential organelles is required for successful cell division. The essential budding yeast myosin V Myo2 actively segregates most organelles along polarized actin cables [1, 2]. The mechanism of mitochondrial segregation remains controversial, with movement driven by actin polymerization [3–5], movement driven by association with transported cortical endoplasmic reticulum (ER)...
The biological role of many nonessential tRNA modifications outside of the anticodon remains elusive despite their evolutionary conservation. We show here that m 7 G 46 methyltransferase Trm8p/Trm82p acts as a hub of synthetic interactions with several tRNA modification enzymes, resulting in temperature-sensitive growth. Analysis of three double mutants indicates reduced levels of...
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