The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Herein, a ratiometric turn-on fluorescent probe for sensitive detection of biothiols was designed. The probe consisted of two parts: one was rhodamine B serving as a fluorescence reference, and the other was coumarin derivative as the responsive fluorophore with an acrylate group for biothiols recognition. The response was based on the mechanism of Michael addition and intramolecular cyclization reaction,...
A microfabricated three-electrode on-chip device with a vibration motor for stripping voltammetric detection of Cd 2+ and Pb 2+ ions is introduced. Gold electrodes on a glass substrate are utilized as the counter electrode directly, as well as the working electrode with bismuth coating. The reference electrode is achieved by painting silver/silver chloride layer on the gold layer....
Core/shell/shell structured Fe3O4/SiO2/Gd2O(CO3)2 nanoparticles were successfully synthesized. Their properties as a new type of T1–T2 dual model contrast agent for magnetic resonance imaging were investigated. Due to the introduce of a separating SiO2 layer, the magnetic coupling between Gd2O(CO3)2 and Fe3O4 could be modulated by the thickness of SiO2 layer and produce appropriate T1 and T2 signal...
Enzyme linked aptamer assay (ELAA) uses an aptamer as recognition element and enzyme as signal readout element for establishing different kinds of aptasensors. We reported herein a high-throughput colorimetric aptasensor based on ELAA only requiring a single aptamer sequence for cocaine detection. An anti-cocaine aptamer was cleaved into two fragments, one of which was immobilized on a DNA-BIND 96-well...
A simple and label-free aptasensor for sensitive and specific detection of cocaine was developed by measuring the change in electrochemical impedance spectra (EIS), based on the formation of a supramolecular aptamer fragments/substrate complex. An anticocaine aptamer was divided into two fragments, Cx and Cy. Three different sensing interfaces, called Au/Cx5S/MCE, Au/Cy3S/MCE and Au/Cy5S/MCE, were...
To solve the problem of low selectivity of current immunochromatographic assay (ICA) for aflatoxin B 1 (AFB 1 ) alone detection, a novel selective ICA was developed here. With very high selectivity, a new AFB 1 monoclonal antibody (MAb) 3G1 was preparaed by immunizing Balb/c mice with aflatoxin B 2a -BSA (AFB 2a -BSA) rather than AFB 1 -BSA used in other...
In this paper, a compact and inexpensive light emitting diode induced fluorescence (LED-IF) detector with simplified optical configuration was developed and assembled in an integrated microfluidic device for microscale electrophoresis. The facile detector mainly consisted of an LED, a focusing pinhole, an emission filter and a photodiode, and was encapsulated in the upper layer of an aluminum alloy...
A Fe 2 O 3 @Au core/shell nanoparticle-based electrochemical DNA biosensor was developed for the amperometric detection of Escherichia coli (E. coli). Magnetic Fe 2 O 3 @Au nanoparticles were prepared by reducing HAuCl 4 on the surfaces of Fe 2 O 3 nanoparticles. This DNA biosensor is based on a sandwich detection strategy, which involves capture...
Sudan I monoclonal antibodies (Mabs) were prepared by hybridoma technique and firstly used to develop a Sudan I immunosensor by immobilizing the Mabs on a gold electrode. o-Mercaptobenzoic acid (MBA) was covalently conjugated on the gold electrode to form a self-assembled monolayer (SAM). The immobilization of Sudan I Mabs to the SAM was carried out through a stable acyl amino ester intermediate generated...
A novel matrix, gold nanoparticles–bacterial cellulose nanofibers (Au–BC) nanocomposite was developed for enzyme immobilization and biosensor fabrication due to its unique properties such as satisfying biocompatibility, good conductivity and extensive surface area, which were inherited from both gold nanoparticles (AuNPs) and bacterial cellulose nanofibers (BC). Heme proteins such as horseradish peroxidase...
The process of ultrasound-assisted emulsification–microextraction (USAEME) was successfully applied for the first time for the extraction and pre-concentration of trace cadmium from water samples, followed by flame atomic absorption spectrometry (FAAS). In the proposed approach, sodium diethyldithiocarbamate trihydrate solution (NaDDTC·3H 2 O) was used as a chelating agent and carbon tetrachloride...
A rapid, specific and sensitive method for assay of Escherichia coli (E. coli) using biofunctional magnetic nanoparticles (BMNPs) in combination with adenosine triphosphate (ATP) bioluminescence was proposed. The BMNPs were fabricated by immobilizing a specific anti-E. coli antibody on the surface of amine-functionalized magnetic nanoparticles (about 20nm in diameter), and then was applied to capture...
A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by...
The effect of Ce(III) on the morphology and structure of lead dioxide (PbO 2 ) modified electrode was studied in this paper. The results obtained by cyclic voltammetry (CV), X-ray diffractometion (XRD) and scanning tunneling micrograph (STM) indicated that the Ce-doped PbO 2 film consisted of a mixture of α- and β-PbO 2 crystallographic phases and the content of α-phase was...
In this paper, multi-wall carbon nanotubes fuctionalized with carboxylic groups modified electrode (MWNT-COOH CME) was fabricated. This chemically modified electrode (CME) can be used as the working electrode in the liquid chromatography for the determination of 6-mercaptopurine (6-MP). The results indicate that the CME exhibits efficiently electrocatalytic oxidation for 6-MP with relatively high...
Recent research on the pathology and treatment of Parkinson's disease (PD) necessitates the development of methods for analysis of dopamine (DA), an electroactive substance whose content reduces in patients with PD. The concentration variation of dopamine in an experimental Parkinsonian animal model and a treatment model was studied in this paper. For the purpose of detecting dopamine sensitively,...
The carbon film based ring-disk dual electrodes in the thin-layer radial flow cell are used as the dual electrochemical detector (DECD) for liquid chromatography (LC) to determine the monoamine neurotransmitters. Cyclic voltammetric experiments show there has great difference in the reversibility of the oxidative reactions of dopamine and ascorbate. Therefore the ring-disk dual electrode arrangement...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.