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This paper reports on a biomimetic microskeleton actuated by muscle fibers (figure 1(a)). The 3D microskeleton is based on concatenated rib elements fabricated by two-photon polymerization of a custom-made hydrogel (figure 1(b)). For the first time, micrometer-sized skeletons were fabricated, overgrown with functional muscle fibers, and contracted at significant amplitudes. Compared to planar biomimetic...
Transmembrane proteins functioning as the cellular signaling gateway are the major molecular targets of commercial drug therapy. HEK293T cells are world widely used cell based-platform for these proteins analysis. We loaded the photo-curable hydrogel into the HEK293T cells, treated with UV through the micron photo-mask, and fabricated the cell membrane fiber. The activity of ion channels on the surface...
Microenvironmental biophysical stimuli influence diverse cellular functions, such as directional motility and stem cell differentiation. Previously, researchers have tuned the linear stiffness of microposts to investigate cell mechanobiological processes and direct cellular behavior; however, microposts suffer from an inherent, yet critical drawback — regulation of micropost stiffness is fundamentally...
This paper describes a microfluidic device for a high through-put ion channel recordings, in which planar bilayer lipid membranes (BLMs) are spanning across a nanometer-sized poly(p-xylylene) (parylene) pore (Figure 1). To make the nanopore, a micrometer-sized pore of parylene was prepared by photolithography, and then parylene was additionally deposited on to the micropore with the isotropic vaporization...
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