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[NiFeSe] hydrogenases are a subgroup of the large family of [NiFe] hydrogenases in which a selenocysteine ligand coordinates the Ni atom at the active site. As observed for other selenoproteins, the [NiFeSe] hydrogenases display much higher catalytic activities than their Cys‐containing homologues. Here, we review the biochemical, catalytic, spectroscopic and structural properties of known [NiFeSe]...
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