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Objective: To determine changes in skeletal muscle metabolism after nerve transection and repair and to correlate metabolic changes with functional recovery. Study Design and Setting: Male Wistar rats were divided into 6 experimental groups plus a control group. The posterior tibial nerve was transected and reapproximated. At varying times after surgery (1, 2, 4, 6, or 8 weeks) animals were sacrificed,...
Calmodulin (CaM) is a calcium binding protein that is known to regulate over 30 different proteins. CaM (148 amino acid residues) contains nine methionines (Met), which form hydrophobic patches that play a crucial role in the binding of Ca-CaM to its target proteins. By use of an E. coli expression system and a strain auxotrophic for Met, it is possible to introduce a variety of unnatural Met analogues...
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