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The in vitro activity of human recombinant β‐secretase (BACE1) was studied using a fluorogenic substrate based on the cleavage site for the enzyme in the Swedish mutation of amyloid precursor protein. The enzyme was inhibited by a control peptide inhibitor with good repeatability. The enzyme preparation comprised a mixture of pro‐enzyme or zymogen and mature enzyme whereby the pro‐enzyme sequence...
A 26 kDa basic subunit of 13S globulin has been purified from grains of common buckwheat (Fagopyrum esculentum Moench). The amino acid composition of the protein closely matches the W.H.O. recommended values for a nutritionally balanced protein. The sequence of 17 N-terminal amino acid residues of the protein revealed 73.3 and 66.7% homology with soya bean glycinin and pea legumin, respectively.
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