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Persistent activation of the inflammatory response contributes to the development of inflammatory bowel diseases, which increase the risk of colorectal cancer. We aimed to identify microRNAs that regulate inflammation during the development of ulcerative colitis (UC) and progression to colitis-associated colon cancer (CAC).We performed a quantitative polymerase chain reaction analysis to measure microRNAs...
Fully unadenylylated glutamine synthetase (GS) from the endophytic bacterium Azospirillum brasilense Sp245 was isolated and purified. The enzyme was electrophoretically homogeneous and contained strongly bound metal ions, which could not be removed by dialysis. Mn2+, Mg2+, and Co2+ were found to be effective in supporting biosynthetic activity of the A. brasilense GS. Some kinetic properties of Mn...
Using Omegon-Km mutagenesis, sixAzospirillum brasilenseSp245 mutant derivatives lacking the capability to synthesize either one of the two major O-specific polysaccharides (O-PSs) were constructedin vivo.In all of the Lps mutants obtained, single Omegon-Km insertions were shown to be located on an indigenous plasmid DNA with molecular weight 120 MDa (p120). Physical and immunochemical analyses revealed...
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