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Cytochrome bd is one of the two terminal ubiquinol oxidases in the respiratory chain of Escherichia coli catalyzing reduction of O 2 to H 2 O. The enzyme is expressed under low oxygen tension; due to high affinity for O 2 it is isolated mainly as a stable oxygenated complex. Direct measurement of O 2 binding to heme d in the one-electron reduced isolated enzyme gives...
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