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Genomic studies revealed the absence of glutaminyl-tRNA synthetase and/or asparaginyl-tRNA synthetase in many bacteria and all known archaea. In these microorganisms, glutaminyl-tRNA Gln (Gln-tRNA Gln ) and/or asparaginyl-tRNA Asn (Asn-tRNA Asn ) are synthesized via an indirect pathway involving side chain amidation of misacylated glutamyl-tRNA Gln (Glu-tRNA...
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