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F1-ATPase is a unique enzyme in terms of its rotational catalytic activity. The smallest unit showing this property is the α3β3γ complex (351 kDa). For investigation of such a huge system by means of solution NMR, we have explored a suitable NMR method using F1-ATPase subcomplexes from a thermophilic Bacillus PS3 including an α3β3 hexamer (319 kDa). Pulse sequences for large molecules, effects of...
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