Miwa Sugiura

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Probing the role of arginine 323 of the D1 protein in photosystem II function

Miwa Sugiura, Tomonori Taniguchi, Nanami Tango, Makoto Nakamura, more

Physiologia Plantarum > 171 > 2 > 183 - 199

The Mn₄CaO₅ cluster of photosystem II (PSII) advances sequentially through five oxidation states (S₀ to S₄). Under the enzyme cycle, two water molecules are oxidized, O₂ is generated and four protons are released into the lumen. Umena et al. (2011) have proposed that, with other charged amino acids, the R323 residue of the D1 protein could contribute to regulate a proton egress pathway from the Mn...

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Consequences of structural modifications in cytochrome b₅₅₉ on the electron acceptor side of Photosystem II

Makoto Nakamura, Alain Boussac, Miwa Sugiura

Photosynthesis Research > 2019 > 139 > 1-3 > 475-486

Cytb₅₅₉ in Photosystem II is a heterodimeric b-type cytochrome. The subunits, PsbE and PsbF, consist each in a membrane α-helix. Mutants were previously designed and studied in Thermosynechococcus elongatus (Sugiura et al., Biochim Biophys Acta 1847:276–285, 2015) either in which an axial histidine ligand of the haem-iron was substituted for a methionine, the PsbE/H23M mutant in which the haem was...

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Corrigendum to "Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of photosystem II in Thermosynechococcus elongatus"

Miwa Sugiura, Yui Ozaki, Fabrice Rappaport, Alain Boussac

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2016 > 1857 > 12 > 1943-1948

Two mutants, D1-H198Q and D1-H198A, have been previously constructed in Thermosynechococcus elongatus with the aim at modifying the redox potential of the P₆₈₀^•+/P₆₈₀ couple by changing the axial ligand of P_D1, one the two chlorophylls of the Photosystem II primary electron donor [Sugiura et al., Biochim. Biophys. Acta 1777 (2008) 331–342]. However, after the publication of this work it was pointed...

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Electron transfer pathways from the S2-states to the S3-states either after a Ca2+/Sr2+ or a Cl−/I− exchange in Photosystem II from Thermosynechococcus elongatus

Alain Boussac, A. William Rutherford, Miwa Sugiura

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2015 > 1847 > 6-7 > 576-586

)′ split EPR signal upon near-infrared illumination. In Sr/Cl-PSII, the high spin (S=5/2) form of S₂ shows a marked heterogeneity with a g=4.3 form generated at low temperature that converts to a relaxed form at g=4.9 at higher temperatures. The high spin g=4.9 form can then progress to the EPR detectable form of S₃ at temperatures as low as 180K whereas the low spin (S=1/2) S₂-state can only advance...

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Assembly of oxygen-evolving Photosystem II efficiently occurs with the apo-Cytb559 but the holo-Cytb559 accelerates the recovery of a functional enzyme upon photoinhibition

Miwa Sugiura, Makoto Nakamura, Kazumi Koyama, Alain Boussac

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2015 > 1847 > 2 > 276-285

Cytb₅₅₉ in Photosystem II is a heterodimeric b-type cytochrome. The subunits, PsbE and PsbF, consist each in a membrane α-helix. Roles for Cytb₅₅₉ remain elusive. In Thermosynechococcus elongatus, taking advantage of the robustness of the PSII variant with PsbA3 as the D1 subunit (WT*3), 4 mutants were designed hoping to get mutants nevertheless the obligatory phototrophy of this cyanobacterium. In...

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The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II

Miwa Sugiura, Yui Ozaki, Masato Nakamura, Nicholas Cox, more

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2014 > 1837 > 12 > 1922-1931

The main cofactors of Photosystem II (PSII) are borne by the D1 and D2 subunits. In the thermophilic cyanobacterium Thermosynechococcus elongatus, three psbA genes encoding D1 are found in the genome. Among the 344 residues constituting the mature form of D1, there are 21 substitutions between PsbA1 and PsbA3, 31 between PsbA1 and PsbA2, and 27 between PsbA2 and PsbA3. In a previous study (Sugiura...

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Some Photosystem II properties depending on the D1 protein variants in Thermosynechococcus elongatus

Miwa Sugiura, Alain Boussac

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2014 > 1837 > 9 > 1427-1434

Cyanobacteria have multiple psbA genes encoding PsbA, the D1 reaction center protein of the Photosystem II complex which bears together with PsbD, the D2 protein, most of the cofactors involved in electron transfer reactions. The thermophilic cyanobacterium Thermosynechococcus elongatus has three psbA genes differently expressed depending on the environmental conditions. Among the 344 residues constituting...

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Variants of photosystem II D1 protein in Thermosynechococcus elongatus

Miwa Sugiura, Alain Boussac

Research on Chemical Intermediates > 2014 > 40 > 9 > 3219-3229

Cyanobacteria have several psbA genes encoding PsbA, the D1 reaction center protein of the photosystem II (PSII) complex which bears, with PsbD, the D2 protein, most of the cofactors involved in electron-transfer reactions. The thermophilic cyanobacterium Thermosynechococcus elongatus has three psbA genes differently expressed depending on the environmental conditions. Among the 344 residues constituting...

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Photosynthesis and artificial photosynthesis research

Mamoru Nango, Miwa Sugiura

Research on Chemical Intermediates > 2014 > 40 > 9 > 3163-3168

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Modification of the pheophytin redox potential in Thermosynechococcus elongatus Photosystem II with PsbA3 as D1

Miwa Sugiura, Chizuko Azami, Kazumi Koyama, A. William Rutherford, more

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2014 > 1837 > 1 > 139-148

In Photosystem II (PSII) of the cyanobacterium Thermosynechococcus elongatus, glutamate 130 in the high-light variant of the D1-subunit (PsbA3) was changed to glutamine in a strain lacking the two other genes for D1, psbA₁ and psbA₂. The resulting PSII (PsbA3/Glu130Gln) was compared with those from the “native” high-light (PsbA3-PSII) and low-light (PsbA1-PSII) variants, which differ by 21 amino acid...

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Crystal structure at 1.5Å resolution of the PsbV2 cytochrome from the cyanobacterium Thermosynechococcus elongatus

Michihiro Suga, Thanh-Lan Lai, Miwa Sugiura, Jian-Ren Shen, more

FEBS Letters > 2013 > 587 > 19 > 3267-3272

PsbV2 is a c-type cytochrome present in a very low abundance in the thermophilic cyanobacterium Thermosynechococcus elongatus. We purified this cytochrome and solved its crystal structure at a resolution of 1.5Å. The protein existed as a dimer in the crystal, and has an overall structure similar to other c-type cytochromes like Cytc ₆ and Cytc ₅₅₀ , for example. However, the 5th and...

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The Tll0287 protein is a hemoprotein associated with the PsbA2-Photosystem II complex in Thermosynechococcus elongatus

Alain Boussac, Kazumi Koyama, Miwa Sugiura

BBA - Bioenergetics > 2013 > 1827 > 10 > 1174-1182

Cyanobacteria have multiple psbA genes encoding PsbA, the D1 reaction center protein of the Photosystem II (PSII) complex. The thermophilic cyanobacterium Thermosynechococcus elongatus has three psbA genes differently expressed depending on the environmental conditions. Among the 344 residues of PsbA, there are 21 substitutions between PsbA1 and PsbA3, 31 between PsbA1 and PsbA2 and 27 between PsbA2...

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Influence of the PsbA1/PsbA3, Ca2+/Sr2+ and Cl−/Br− exchanges on the redox potential of the primary quinone QA in Photosystem II from Thermosynechococcus elongatus as revealed by spectroelectrochemistry

Yuki Kato, Tadao Shibamoto, Shoichi Yamamoto, Tadashi Watanabe, more

BBA - Bioenergetics > 2012 > 1817 > 11 > 1998-2004

Ca ²⁺ and Cl ⁻ ions are essential elements for the oxygen evolution activity of photosystem II (PSII). It has been demonstrated that these ions can be exchanged with Sr ²⁺ and Br ⁻ , respectively, and that these ion exchanges modify the kinetics of some electron transfer reactions at the Mn ₄ Ca cluster level (Ishida et al., J. Biol. Chem. 283 (2008) 13330–13340)...

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Deactivation processes in PsbA1-Photosystem II and PsbA3-Photosystem II under photoinhibitory conditions in the cyanobacterium Thermosynechococcus elongatus

Shogo Ogami, Alain Boussac, Miwa Sugiura

BBA - Bioenergetics > 2012 > 1817 > 8 > 1322-1330

The sensitivity to high light conditions of Photosystem II with either PsbA1 (WT*1) or PsbA3 (WT*3) as the D1 protein was studied in whole cells of the thermophilic cyanobacterium Thermosynechococcus elongatus. When the cells are cultivated under high light conditions the following results were found: (i) The O ₂ evolution activity decreases faster in WT*1 cells than in WT*3 cells both in...

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Probing the role of chloride in Photosystem II from Thermosynechococcus elongatus by exchanging chloride for iodide

Alain Boussac, Naoko Ishida, Miwa Sugiura, Fabrice Rappaport

BBA - Bioenergetics > 2012 > 1817 > 5 > 802-810

The active site for water oxidation in Photosystem II (PSII) goes through five sequential oxidation states (S ₀ to S ₄ ) before O ₂ is evolved. It consists of a Mn ₄ CaO ₅ cluster and Tyr _Z , a redox-active tyrosine residue. Chloride ions have been known for long time to be required for the function of the enzyme. However, X-ray data have shown that...

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Distribution of the Cationic State over the Chlorophyll Pair of the Photosystem II Reaction Center

Keisuke Saito, Toyokazu Ishida, Miwa Sugiura, Keisuke Kawakami, more

Journal of the American Chemical Society > 2011 > 133 > 36 > 14379 - 14388

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Semiquinone–Iron Complex of Photosystem II: EPR Signals Assigned to the Low-Field Edge of the Ground State Doublet of Q _A ^•– Fe ²⁺ and Q _B ^•– Fe ²⁺

Arezki Sedoud, Nicholas Cox, Miwa Sugiura, Wolfgang Lubitz, more

Biochemistry > 2011 > 50 > 27 > 6012 - 6021

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Effect of Ca ²⁺ /Sr ²⁺ Substitution on the Electronic Structure of the Oxygen-Evolving Complex of Photosystem II: A Combined Multifrequency EPR, ⁵⁵ Mn-ENDOR, and DFT Study of the S ₂ State

Nicholas Cox, Leonid Rapatskiy, Ji-Hu Su, Dimitrios A. Pantazis, more

Journal of the American Chemical Society > 2011 > 133 > 10 > 3635 - 3648

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Probing the quinone binding site of Photosystem II from Thermosynechococcus elongatus containing either PsbA1 or PsbA3 as the D1 protein through the binding characteristics of herbicides

Alain Boussac, Miwa Sugiura, Fabrice Rappaport

BBA - Bioenergetics > 2011 > 1807 > 1 > 119-129

The main cofactors involved in Photosystem II (PSII) oxygen evolution activity are borne by two proteins, D1 (PsbA) and D2 (PsbD). In Thermosynechococcus elongatus, a thermophilic cyanobacterium, the D1 protein is predominantly encoded by either the psbA ₁ or the psbA ₃ gene, the expression of which depends on the environmental conditions. In this work, the Q _B site properties...

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Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus

Miwa Sugiura, Sayo Harada, Takashi Manabe, Hidenori Hayashi, more

BBA - Bioenergetics > 2010 > 1797 > 8 > 1546-1554

A deletion mutant that lacks the Psb30 protein, one of the small subunits of Photosystem II, was constructed in a Thermosynechococcus elongatus strain in which the D1 protein is expressed from the psbA ₃ gene (WT*). The ΔPsb30 mutant appears more susceptible to photodamage, has a cytochrome b ₅₅₉ that is converted into the low potential form, and probably also lacks the PsbY subunit...

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Search results for: Miwa Sugiura

Probing the role of arginine 323 of the D1 protein in photosystem II function

Consequences of structural modifications in cytochrome b₅₅₉ on the electron acceptor side of Photosystem II

Corrigendum to "Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of photosystem II in Thermosynechococcus elongatus"

Electron transfer pathways from the S2-states to the S3-states either after a Ca2+/Sr2+ or a Cl−/I− exchange in Photosystem II from Thermosynechococcus elongatus

Assembly of oxygen-evolving Photosystem II efficiently occurs with the apo-Cytb559 but the holo-Cytb559 accelerates the recovery of a functional enzyme upon photoinhibition

The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II

Some Photosystem II properties depending on the D1 protein variants in Thermosynechococcus elongatus

Variants of photosystem II D1 protein in Thermosynechococcus elongatus

Photosynthesis and artificial photosynthesis research

Modification of the pheophytin redox potential in Thermosynechococcus elongatus Photosystem II with PsbA3 as D1

Crystal structure at 1.5Å resolution of the PsbV2 cytochrome from the cyanobacterium Thermosynechococcus elongatus

The Tll0287 protein is a hemoprotein associated with the PsbA2-Photosystem II complex in Thermosynechococcus elongatus

Influence of the PsbA1/PsbA3, Ca2+/Sr2+ and Cl−/Br− exchanges on the redox potential of the primary quinone QA in Photosystem II from Thermosynechococcus elongatus as revealed by spectroelectrochemistry

Deactivation processes in PsbA1-Photosystem II and PsbA3-Photosystem II under photoinhibitory conditions in the cyanobacterium Thermosynechococcus elongatus

Probing the role of chloride in Photosystem II from Thermosynechococcus elongatus by exchanging chloride for iodide

Distribution of the Cationic State over the Chlorophyll Pair of the Photosystem II Reaction Center

Semiquinone–Iron Complex of Photosystem II: EPR Signals Assigned to the Low-Field Edge of the Ground State Doublet of Q _A ^•– Fe ²⁺ and Q _B ^•– Fe ²⁺

Effect of Ca ²⁺ /Sr ²⁺ Substitution on the Electronic Structure of the Oxygen-Evolving Complex of Photosystem II: A Combined Multifrequency EPR, ⁵⁵ Mn-ENDOR, and DFT Study of the S ₂ State

Probing the quinone binding site of Photosystem II from Thermosynechococcus elongatus containing either PsbA1 or PsbA3 as the D1 protein through the binding characteristics of herbicides

Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus

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