Search results for: Sum P. Lee

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article

Liver fluke-induced hepatic oxysterols stimulate DNA damage and apoptosis in cultured human cholangiocytes

Apinya Jusakul, Watcharin Loilome, Nisana Namwat, W. Geoffrey Haigh, more

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis > 2012 > 731 > 1-2 > 48-57

Oxysterols are cholesterol oxidation products that are generated by enzymatic reactions through cytochrome P450 family enzymes or by non-enzymatic reactions involving reactive oxygen and nitrogen species. Oxysterols have been identified in bile in the setting of chronic inflammation, suggesting that biliary epithelial cells are chronically exposed to these compounds in certain clinical settings. We...

article

Hepatic Free Cholesterol Accumulates in Obese, Diabetic Mice and Causes Nonalcoholic Steatohepatitis

Derrick M. Van Rooyen, Claire Z. Larter, W. Geoffrey Haigh, Matthew M. Yeh, more

Gastroenterology > 2011 > 141 > 4 > 1393-1403.e5

Type 2 diabetes and nonalcoholic steatohepatitis (NASH) are associated with insulin resistance and disordered cholesterol homeostasis. We investigated the basis for hepatic cholesterol accumulation with insulin resistance and its relevance to the pathogenesis of NASH.Alms1 mutant (foz/foz) and wild-type NOD.B10 mice were fed high-fat diets that contained varying percentages of cholesterol; hepatic...

chapter

Gallstones

Cynthia W. Ko, Sum P. Lee

Atlas of Gastroenterology > Gastrointestinal Diseases > 582 - 591

This chapter contains sections titled: Classification of gallstones Pathogenesis of cholesterol gallstones Diagnosis of gallstones Management of gallstones

article

Gallbladder Epithelial Cells that Engraft in Mouse Liver Can Differentiate into Hepatocyte-Like Cells

Sum P. Lee, Christopher E. Savard, Rahul Kuver

The American Journal of Pathology > 2009 > 174 > 3 > 842-853

We tested the hypothesis that well-differentiated gallbladder epithelial cells (GBECs) are capable of engrafting and surviving in murine liver and acquire phenotypic characteristics of hepatocytes. GBECs isolated from transgenic mice that constitutively express green fluorescent protein (GFP) were either cultured before transplantation or transplanted immediately following isolation. Recipient mice...

article

S1793 Analysis of Oxysterols in the Rabbit Gallbladder in the Experimental Cholecystitis

Seok Ho Dong, Christopher E. Savard, Rahul P. Kuver, Sum P. Lee

Gastroenterology > 2008 > 134 > 4 Supplement 1 > A-270

article

Hepatic free fatty acids accumulate in experimental steatohepatitis: Role of adaptive pathways

Claire Z. Larter, Matthew M. Yeh, W. Geoffrey Haigh, Jacqueline Williams, more

Journal of Hepatology > 2008 > 48 > 4 > 638-647

We determined the effects of dietary lipid composition on steatohepatitis development with particular attention to the nature of lipid molecules that accumulate in the liver and pathways of hepatic triglyceride synthesis.Mice were fed methionine and choline deficient (MCD) diets supplemented with 20% fat as lard (saturated) or olive oil (monounsaturated), for 3 weeks.Irrespective of dietary lipid...

article

Insulin Resistance and Incident Gallbladder Disease in Pregnancy

Cynthia W. Ko, Shirley A.A. Beresford, Scott J. Schulte, Sum P. Lee

Clinical Gastroenterology and Hepatology > 2008 > 6 > 1 > 76-81

Background & Aims: Insulin resistance is associated with prevalent gallstones, but its effect on initial gallstone formation is not well-understood. Methods: We conducted a nested case-control study to examine whether insulin resistance is a risk factor for initial gallbladder sludge and stone formation during pregnancy. Cases were 205 women with new gallbladder sludge and stones during pregnancy...

article

Paclitaxel Interrupts TGF-β1 Signaling Between Gallbladder Epithelial Cells and Myofibroblasts

Ho-Soon Choi, Christopher E. Savard, Jae-Woon Choi, Rahul Kuver, more

Journal of Surgical Research > 2007 > 141 > 2 > 183-191

The cellular and molecular mechanisms of fibrogenesis in the extrahepatic biliary epithelium are not known. Transforming growth factor-β1 (TGF-β1) is a cytokine implicated in signaling pathways that mediate collagen formation. An observation that paclitaxel (PT), applied topically into the rat common bile duct, inhibited stricture formation led us to hypothesize that PT’s effects might be due to interruption...

article

Incidence and Predictors of Hepatocellular Carcinoma in Patients With Cirrhosis

George N. Ioannou, Meaghan F. Splan, Noel S. Weiss, George B. McDonald, more

Clinical Gastroenterology and Hepatology > 2007 > 5 > 8 > 938-945.e4

Background & Aims: Independent predictors of hepatocellular carcinoma in patients with cirrhosis are not well established. Methods: We created a cohort of 2126 patients (41% with hepatitis C virus [HCV] infection) who sought care from all Veterans Affairs health care centers in the northwest United States from 1994 to 2005 and who had a diagnosis of cirrhosis recorded in inpatient or outpatient...

article

Liver-mediated clearance of peripheral amyloid-beta (1-40)

J. Jacob Kulstad, Christopher E. Savard, Sum P. Lee, Suzanne Craft, more

Alzheimer's & Dementia: The Journal of the Alzheimer's Association > 2006 > 2 > 3 Supplement > S237-S238

article

The production of oxysterols in bile by activated human leukocytes

W. Geoffrey Haigh, Thomas Wong, Sum P. Lee

Biochemical and Biophysical Research Communications > 2006 > 343 > 2 > 467-469

Oxysterols are naturally occurring intermediates in the conversion of cholesterol to bile acids, the major route for elimination of cholesterol. Additionally, they are important signaling agents, particularly in control of cholesterol synthesis; however, some species also are cytotoxic and carcinogenic. Oxysterols in plasma, contained in oxidized low-density lipoprotein, are strongly correlated with...

article

Interactions between sphingomyelin and cholesterol in low density lipoproteins and model membranes

Andrew J. Guarino, Sum P. Lee, Steven P. Wrenn

Journal of Colloid And Interface Science > 2006 > 293 > 1 > 203-212

This work examines three related, but previously unexplored, aspects of membrane biophysics and colloid science in the context of atherosclerosis. First, we show that sphingomyelinase (SMase)-induced aggregation of low density lipoproteins (LDLs), coupled with LDL exposure to cholesterol esterase (CEase), results in nucleation of cholesterol crystals, long considered the hallmark of atherosclerosis...

article

Dietary Carbohydrates and Gallstones: Is There a Link?

Cynthia W. Ko, Sum P. Lee

Gastroenterology > 2005 > 129 > 1 > 373-375

article

Biliary Sludge Is Formed by Modification of Hepatic Bile by the Gallbladder Mucosa

Cynthia W. Ko, Scott J. Schulte, Sum P. Lee

Clinical Gastroenterology and Hepatology > 2005 > 3 > 7 > 672-678

Background & Aims We studied 22 patients with symptomatic microlithiasis to determine whether a contributory role of the gallbladder in the early stage of cholesterol gallstone formation exists. We compared the merits of different methods (ultrasonography and microscopy) and sources (hepatic or gallbladder) of bile samples for diagnosing microlithiasis. Methods: Paired hepatic and gallbladder...

article

Combined Interaction of Phospholipase C and Apolipoprotein A-I with Small Unilamellar Lecithin-Cholesterol Vesicles: Influence of Apolipoprotein A-I Concentration and Vesicle Composition ^†

Manasa V. Gudheti, Sum P. Lee, Dganit Danino, Steven P. Wrenn

Biochemistry > 2005 > 44 > 19 > 7294 - 7304

article

Contribution of metabolic factors to alanine aminotransferase activity in persons with other causes of liver disease

George N. Ioannou, Noel S. Weiss, Edward J. Boyko, Steven E. Kahn, more

Gastroenterology > 2005 > 128 > 3 > 627-635

Background & Aims: Nonalcoholic fatty liver disease has been defined by the presence of hepatic steatosis in the absence of other chronic liver diseases. We sought to determine whether obesity, insulin resistance, and the metabolic syndrome, which are the main risk factors for nonalcoholic fatty liver disease, are associated with similar elevations in serum alanine aminotransferase activity in...

article

Aggregation kinetics of low density lipoproteins upon exposure to sphingomyelinase

Andrew J. Guarino, Sum P. Lee, Thomas N. Tulenko, Steven P. Wrenn

Journal of Colloid And Interface Science > 2004 > 279 > 1 > 109-116

The response-to-retention hypothesis in atherosclerosis states that subendothelial retention of cholesterol-rich, atherogenic lipoproteins is the central pathogenic event that is both necessary and sufficient to provoke lesion initiation in an otherwise normal artery. Sphingomyelinase-induced aggregation of low density lipoproteins (LDL) is known to facilitate LDL retention, and the only available...

article

Calcium binding to biliary mucins is dependent on sodium ion concentration: relevance to cystic fibrosis

Rahul Kuver, Sum P. Lee

Biochemical and Biophysical Research Communications > 2004 > 314 > 2 > 330-334

Because hypersecretion of gallbladder (GB) mucus occurs in gallstone formation and because binding of Ca ² ⁺ to biliary lipids only accounts for 50% of the total Ca ² ⁺ in GB bile, we investigated the binding of Ca ² ⁺ to human biliary mucin. Biliary mucin was purified from GB bile and binding to Ca ² ⁺ studied. Scatchard plot analysis suggested two...

article

Estimating the size of laterally phase separated cholesterol domains in model membranes with Forster resonance energy transfer: a simulation study

Gregory M. Troup, Thomas N. Tulenko, Sum P. Lee, Steven P. Wrenn

Colloids and Surfaces B: Biointerfaces > 2004 > 33 > 1 > 57-65

In this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations...

article

Interaction of apolipoprotein A-I with lecithin-cholesterol vesicles in the presence of phospholipase C

Manasa V. Gudheti, Yamaira I. Gonzalez, Sum P. Lee, Steven P. Wrenn

BBA - Molecular and Cell Biology of Lipids > 2003 > 1635 > 2-3 > 127-141

Here we study the anti-nucleating mechanism of apolipoprotein A-I (apo A-I) on model biliary vesicles in the presence of phospholipase C (PLC) utilizing dynamic light scattering (DLS), steady-state fluorescence spectroscopy, cryogenic transmission electron microscopy (cryo-TEM), and UV/Vis spectroscopy. PLC induces aggregation of cholesterol-free lecithin vesicles from an initial, average size of...

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INFONA - science communication portal

Search results for: Sum P. Lee

Liver fluke-induced hepatic oxysterols stimulate DNA damage and apoptosis in cultured human cholangiocytes

Hepatic Free Cholesterol Accumulates in Obese, Diabetic Mice and Causes Nonalcoholic Steatohepatitis

Gallstones

Gallbladder Epithelial Cells that Engraft in Mouse Liver Can Differentiate into Hepatocyte-Like Cells

S1793 Analysis of Oxysterols in the Rabbit Gallbladder in the Experimental Cholecystitis

Hepatic free fatty acids accumulate in experimental steatohepatitis: Role of adaptive pathways

Insulin Resistance and Incident Gallbladder Disease in Pregnancy

Paclitaxel Interrupts TGF-β1 Signaling Between Gallbladder Epithelial Cells and Myofibroblasts

Incidence and Predictors of Hepatocellular Carcinoma in Patients With Cirrhosis

Liver-mediated clearance of peripheral amyloid-beta (1-40)

The production of oxysterols in bile by activated human leukocytes

Interactions between sphingomyelin and cholesterol in low density lipoproteins and model membranes

Dietary Carbohydrates and Gallstones: Is There a Link?

Biliary Sludge Is Formed by Modification of Hepatic Bile by the Gallbladder Mucosa

Combined Interaction of Phospholipase C and Apolipoprotein A-I with Small Unilamellar Lecithin-Cholesterol Vesicles: Influence of Apolipoprotein A-I Concentration and Vesicle Composition ^†

Contribution of metabolic factors to alanine aminotransferase activity in persons with other causes of liver disease

Aggregation kinetics of low density lipoproteins upon exposure to sphingomyelinase

Calcium binding to biliary mucins is dependent on sodium ion concentration: relevance to cystic fibrosis

Estimating the size of laterally phase separated cholesterol domains in model membranes with Forster resonance energy transfer: a simulation study

Interaction of apolipoprotein A-I with lecithin-cholesterol vesicles in the presence of phospholipase C

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